IHC Titre
FACS Titre
Specificity/Comments
(CD31/Pecam-1) (supernatant)
1/400
1/100
1/100
1/200
1/5
1/50
1/10
1/200
1/400
1/200
1/200
1/100
1/30
1/10
1/10
1/10
1/10
1/10
1/10
Isotypes are specified for the MTS/HTS series and others in common use where known
| Antibody | Isotype | IHC Titre |
FACS Titre |
Specificity/Comments |
Image(s) |
| MTS 1 (supernatant) | IgM | pan epithelium | |||
| MTS 5 (supernatant) | IgM | pan epithelium but no perivas spaces | |||
| MTS 6 (MHCII) (supernatant) | IgG2a | MHC II, invariant Ia chain | |||
| MTS 7 (supernatant) | IgM | mTEC and fibroblasts | |||
| MTS 9 (supernatant) | IgM | mTEC and fibroblasts | |||
| MTS 10 (supernatant) | IgM | Medullary epthelium | |
||
| MTS 12 (CD31/Pecam-1) (supernatant) |
IgG2a | Vascular Epi/Endoderm | |||
| MTS 15 (supernatant) | IgG2c | Fibroblast subset, vascular association | |||
| MTS 16 (supernatant) | IgG2a | Extracellular Matrix | |||
| MTS 20 (supernatant) | IgM | rare mTEC subsets/clusters sim 24 | |||
| MTS 23 (Sca 1) | Check Daisy's thesis | ||||
| MTS 24 (supernatant) | IgG2a | rare mTEC subsets/clusters | |
||
| MTS 29 (supernatant) | IgG2a | rare mTEC subsets/clusters dif 24 | |||
| MTS 32 (TSA 2) (supernatant) | IgM | Thymocytes + epithelium | |||
| MTS 33 (ThB) (supernatant) | IgG1 | Cortical thymocytes, Med clusters; T & B cells | |||
| MTS 35 (TSA1) (supernatant) | IgG1 | TSA-1 | |||
| MTS 37 (HSA) (supernatant) | ND | isol med ep | |||
| MTS44 (supernatant) | IgMk | Cortical Ep | |
||
| AIRE | IgG2c | 1/400 |
Small Clusters in medullary regions | ||
| B7.1 | 1/100 |
DC, IIhi mTECs | |||
| CD40 | IgG2a | 1/100 |
DC, IIhi mTECs | ||
| CD3 (145-2C11) (supernatant) | IgG2a | Hamster anti-mouse CD3 | |||
| CD3 (KT3) (supernatant) | Rat anti-mouse CD3 | ||||
| CD28 | hamster | ||||
| CD34 | IgG2a | ||||
| CDR1 (Ly51/6C3) (supernatant) | Cortex/Fibroblasts | ||||
| cytokeratin | polyclnl | 1/200 |
Rabbit anti-pan epithelium | ||
| CD106 (VCAM1) | IgG2a | ||||
| ERTR7 (supernatant) | Fibroblast | ||||
| G8.8 (supernatant) | IgG2aK | Epithelium (EpCAM) | |||
| IgG1 k | |||||
| IL-1R | |||||
| KGFR (FgFR2IIIb) | TECS and fibroblasts? | ||||
| K8 (Troma1) | 1/5 |
Cortical epithelium | |||
| LTBR | IgG2a | 1/50 |
Whole stroma..? | ||
| Mac-1 (CD11b/M1-70) (supernatant) | Macrophages | ||||
| M1/78 | IgM | 1/10 |
Fibroblasts (Forssman) | ||
| Ox6 | IgG1 | 1/200 |
1/400 |
NOD MHCII | |
| PDGFRa | IgG | 1/200 |
1/200 |
Fibroblasts | |
| a Phosphotyrosine | |||||
| TSA-1 | IgG1 | ||||
| VCAM | 1/100 |
Subset cTEC and Endothelium | |||
| HUMAN | |||||
| CDR2 (supernatant) | IgG | Human cortical epithelium (+ fibroblasts) | |||
| HTS-1 (supernatant) | Epithelial cells scattered in subcapsule, cortex and medulla. Epi around but not on HC | ||||
| HTS-2 (supernatant) | Reticular meshwork in cortex and medulla | ||||
| HTS-6 (supernatant) | Subcapsular and subtrabecular region coloc with keratin | ||||
| HTS-8 (supernatant) | Subcapsular, subtrabecular and medullary epithelium | ||||
| CONTROLS | |||||
| IgG1 | Rat non specific isotype control | ||||
| IgG2a | Rat non specific isotype control | ||||
| IgG2b | Rat non specific isotype control | ||||
| IgG2c | Rat non specific isotype control | ||||
| FcR (supernatant) | 1/30 |
Rat anti-FcR. Used mainly for blocking steps | |||
| Normal goat serum | 1/10 |
1/10 |
Used mainly for blocking steps | ||
| Normal rat serum | 1/10 |
1/10 |
Used mainly for blocking steps | ||
| Normal mouse serum | 1/10 |
1/10 |
Used mainly for blocking steps |