Conjugates and Secondaries

Most often these are secondary antibodies but can be used as primaries in some cases also

Conjugate IHC Titre FACS Titre Specificity / Comments
Rat      
a-rat 488 1/200 1/200-300 Rat H&L chains
a-rat 568 1/100 (1/500)?   Rat H&L chains
a-rat FITC 1/50 1/100 Rat H&L chains
a-rat Bio 1/100 1/200 Rat H&L chains
a-rat PE ? ? Rat H&L chains
       
Rabbit      
a-rabbit 647 1/100    
a-rabbit 568 1/400-1/500    
a-rabbit 594 1/400-1/500    
a-rabbit 488 1/300    
       
Human      
CD45F   neat or 1/2  
HLA DR PE   1-10  
UEA1 bio   1/25000  
anti-mouse Ig FITC   1/40 From Pera lab recognises Fab region of mouse Ig
SA CyC   1/500  
EPCAM PCP      
CD45 APC      
       
Streptavidin      
SA Cy5 1/200 1/500  
SA 488 (old) 1/400-100 new?    
SA 568 1/1000 old/1/200    
SA APC 1/1000    
SA PE 1/2000    
SA PerCP   1/100  
       
Isotype secondaries      
IgM biotin 1/1000    
IgG2a FITC 1/80    
IgG2a bio 1/1000    
IgG2c FITC 1/1000    
       
By Fluorochrome
FITC IHC FACS Specificity / Comments
Annexin-V   1/20  
anti-K5 1/50   medullary TECs
B220/CD45R   1/400 B&T cells
CD2   1/900 SRBC
CD3   1/200  
CD4   1/1000  
CD8a   1/200  
CD11b (MAC-1)   1/200 Macrophages
CD11c 1/100 1/200 DC's
CD19   1/800 B cells
CD25   1/200 IL-2 receptor
CD21/CD35   1/800 complement receptors
CD40 1/50    
CD44   1/800  
CD45.1   1/500 Leucocyte common antigen
CD45.2 (IgG2ak)   1/100  
CD62L   1/100  
CD80   1/50 Immature DC?
CD103   1/600  
Gr1 (new)   1/1000 Granulocytes
IgD   1/400  
IgM   1/200  
IFN-gamma   1/400  
6C3 (ly51)   1/50  
Ly6G   ?  
H-2DK   ?  
IgD   ?  
IgG2a FITC 1/80    
IgG2c FITC 1/1000    
Rat IgG2b ?    
Rab Ig 1/30 1/100  
TCR-beta   1/50  
TCR-gamma delta   1/50  
TNF-alpha   1/400  
TSA-1   1/50  
UEA-1 1/400 1/500  
Va2   ?  
       
PE IHC FACS Specificity / Comments
Annexin V   ?  
B220 (CD45R)   1/200  
c-kit   poor  
CD3   1/100  
CD4   1/800  
CD8b.2 (Ly3.2)   1/200  
CD11b   1/400  
CD11c   1/200  
CD19   1/800  
CD25   1/1000  
CD28      
CD43 (Ly-48)   1/200  
CD45 (IgG2bK)   ?  
CD45 (ly5)   1/300  
CD45R/B220   1/200  
CD69   1/1000  
CD62L (L-selectin)   1/200  
CD121a (IL-1R)      
CD152 (CTLA-4)   1/100  
CTLA-4   1/100  
Gr-1   1/1000  
IgM   1/100  
IL-2   1/400  
IL-4   1/100  
IL7R   1/30  
IL-10   ?  
IL-12   ?  
MHCII (IgG2bk)   1/1600  
NK1.1   1/200  
SCA1 (IgG2a)   1/200  
Strep-PE 1/2000    
Va2 TCR   1/300  
       
PerCP IHC FACS Specificity / Comments
CD4   1/1000  
CD45R/B220   1/400  
CD8a   1/100  
Strep-PerCP   1/100  
       
Cy5 IHC FACS Specificity / Comments
CD44   1/800  
Strep-Cy5 1/200 1/500  
       
BIOTIN IHC FACS Specificity / Comments
Annexin V   ?  
CD3e      
CD5 (Ly-1)   1/800  
CD8a   1/800  
CD11c bio 1/100 1/200  
CD19   1/300  
CD23 (IgE FCR)   1/200  
CD24 (HSA)   1/200  
CD31 bio stock 1:20 1/50 1/100 Pecam endothelium
CD44   1/1000  
CD45.1   1/500?  
CD45.2   1/80  
CD90.1 (Thy1.1)   1/100  
Epcam-Bio (G8.8)   1/200  
FAS      
FAS ligand   1/100  
Gr1-bio   1/1000  
H-2Db 1/100    
H-2Kb 1/100    
H-2Kd   1/100  
H-2kk   1/100  
Ig   ? ? species
IL-4   ?  
IL-5   ?  
Iso-bio   1/200  
6C3 (Ly51)-bio 1/100    
Ly51-bio (6C3) 1/200 1/400  
Sca1 bio 1/100 1/100  
Thy 1.2   ?  
UEA1-bio 1/400 1/800  
       
APC IHC FACS Specificity / Comments
c-kit   1/50  
CD4   1/400  
CD8a   1/400  
CD11b   1/800  
CD25 (IL2Ra)   1/400  
CD45-APC   1/100-200  
CD62-L   1/400  
Strep-APC 1/1000    
TCR b   1/100