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Isotypes

Full antibody list here:

Isotypes are specified for the MTS/HTS series and others in common use where known

Antibody Isotype
IHC Titre
FACS Titre
Specificity/Comments
Image(s)
MTS 1 (supernatant) IgM
 
 
pan epithelium  
MTS 5 (supernatant) IgM
 
 
pan epithelium but no perivas spaces  
MTS 6 (MHCII) (supernatant) IgG2a
 
 
MHC II, invariant Ia chain  
MTS 7 (supernatant) IgM
 
 
mTEC and fibroblasts  
MTS 9 (supernatant) IgM
 
 
mTEC and fibroblasts  
MTS 10 (supernatant) IgM
 
 
Medullary epthelium
MTS 12
(CD31/Pecam-1) (supernatant)
IgG2a
 
 
Vascular Epi/Endoderm  
MTS 15 (supernatant) IgG2c
 
 
Fibroblast subset, vascular association  
MTS 16 (supernatant) IgG2a
 
 
Extracellular Matrix  
MTS 20 (supernatant) IgM
 
 
rare mTEC subsets/clusters sim 24  
MTS 23 (Sca 1)       Check Daisy's thesis  
MTS 24 (supernatant) IgG2a
 
 
rare mTEC subsets/clusters image
MTS 29 (supernatant) IgG2a
 
 
rare mTEC subsets/clusters dif 24  
MTS 32 (TSA 2) (supernatant) IgM
 
 
Thymocytes + epithelium  
MTS 33 (ThB) (supernatant) IgG1
 
 
Cortical thymocytes, Med clusters; T & B cells  
MTS 35 (TSA1) (supernatant) IgG1
 
 
TSA-1  
MTS 37 (HSA) (supernatant) ND
 
 
isol med ep  
MTS44 (supernatant) IgMk
 
 
Cortical Ep image
   
 
 
   
AIRE IgG2c
1/400
 
Small Clusters in medullary regions  
B7.1  
 
1/100
DC, IIhi mTECs  
CD40 IgG2a
 
1/100
DC, IIhi mTECs  
CD3 (145-2C11) (supernatant) IgG2a
 
 
Hamster anti-mouse CD3  
CD3 (KT3) (supernatant)  
 
 
Rat anti-mouse CD3  
CD28 hamster
 
 
   
CD34 IgG2a
 
 
   
CDR1 (Ly51/6C3) (supernatant)  
 
 
Cortex/Fibroblasts  
cytokeratin polyclnl
1/200
 
Rabbit anti-pan epithelium  
CD106 (VCAM1) IgG2a
 
 
   
ERTR7 (supernatant)  
 
 
Fibroblast  
G8.8 (supernatant) IgG2aK
 
 
Epithelium (EpCAM)  
IgG1 k  
 
 
   
IL-1R  
 
 
   
KGFR (FgFR2IIIb)  
 
 
TECS and fibroblasts?  
K8 (Troma1)  
1/5
 
Cortical epithelium  
LTBR IgG2a
1/50
 
Whole stroma..?  
Mac-1 (CD11b/M1-70) (supernatant)  
 
 
Macrophages  
M1/78 IgM
1/10
 
Fibroblasts (Forssman)  
Ox6 IgG1
1/200
1/400
NOD MHCII  
PDGFRa IgG
1/200
1/200
Fibroblasts  
a Phosphotyrosine  
 
 
   
TSA-1 IgG1
 
 
   
VCAM  
1/100
 
Subset cTEC and Endothelium  
   
 
 
   
HUMAN  
 
 
   
CDR2 (supernatant) IgG
 
 
Human cortical epithelium (+ fibroblasts)  
HTS-1 (supernatant)  
 
 
Epithelial cells scattered in subcapsule, cortex and medulla. Epi around but not on HC  
HTS-2 (supernatant)  
 
 
Reticular meshwork in cortex and medulla  
HTS-6 (supernatant)  
 
 
Subcapsular and subtrabecular region coloc with keratin  
HTS-8 (supernatant)  
 
 
Subcapsular, subtrabecular and medullary epithelium  
   
 
 
   
CONTROLS  
 
 
   
IgG1  
 
 
Rat non specific isotype control  
IgG2a  
 
 
Rat non specific isotype control  
IgG2b  
 
 
Rat non specific isotype control  
IgG2c  
 
 
Rat non specific isotype control  
   
 
 
   
FcR (supernatant)  
 
1/30
Rat anti-FcR. Used mainly for blocking steps  
Normal goat serum  
1/10
1/10
Used mainly for blocking steps  
Normal rat serum  
1/10
1/10
Used mainly for blocking steps  
Normal mouse serum  
1/10
1/10
Used mainly for blocking steps  

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