RBC Lysis Buffer

Contents:

 

Spleen/BM RBC Lysis

  • Pellet cells (usually Spleen/Bone Marrow suspension) in the centrifuge .
  • Using a yellow capped tube at 1500RPM for 5mins
  • Resuspend in 1mL RBC Lysis buffer for 1 minute
  • Dilute with PBS to inactivate, spin down to a pellet
  • Aspirate and resuspend in PBS

 

Making up RBC Lysis Buffer

  • 4.15 g Ammonium Chloride
  • 50mL 0.1M Tris HCL
  • Make up to 500mL with RO Water
  • Modify pH to 7.5 ±0.2