Lucky Lucrin Preparation Protocol

Contents:

 

10x PBS (Mouse Tonicity)

 

For 4L:

NaCl 348g

NaH 2 PO 4 .2H 2 O 25g

Na 2 HPO 4 *(anhydrous) 91.0g

 

* or Na 2 HPO 4 .2H 2 O 114g

or Na 2 HPO 4 .12H 2 O 229.3g

 

For 20L:

NaCl (870g in 5L) x 2

NaH 2 PO 4 .2H 2 O 125g in 5L

Na 2 HPO 4 * (anhydrous) 454.7g in 5L

Add together to make 20L

 

* or Na 2 HPO 4 .2H 2 O 570g

or Na 2 HPO 4 .12H 2 O 1146.4g

 

10x PBS (Human Tonicity)

 

For 4L:

NaCl 340g

NaH 2 PO 4 .2H 2 O 15.6g

Na 2 HPO 4 * (anhydrous) 42.8g

 

* or Na 2 HPO 4 .2H 2 O 53.65g

or Na 2 HPO 4 .12H 2 O 107.9g

 

N.B. Store at room temperature; do not autoclave or refridgerate