Lucky Lucrin Preparation Protocol
Lucrin preparation should be done in the hood.
For 0.4mg dose in 50uL
If using an alternate source of Lucrin, measure the amount of diluent the Lucrin is suspended in. This amount is X mL (for calculations)
There is 22.5mg of Lucrin in a single vial. Mice require a 0.4mg dose.
22.5mg / X mL = Y (mg/ mL)
(amount of Lucrin in 1 ml of suspension)
0.4mg / Y = Z (ml)
(amount of initial suspension required for a 0.4mg dose for a single animal)
50 - Z = # ml of sterile PBS required to make up suspension to a 50 ml dose per mouse W (ml)
Hence if there are 20 mice requiring doses:
Z ml x 20 = Amount of initial suspension needed
W ml x 20 = Amount of sterile PBS which needs to be added
This gives you the right amount of doses at 50 ml of suspension per mouse (always make up more doses than you need!)
I have a 1273 ml suspension of Lucrin and need to make up 20 doses
22.5 / 1273 = 0.0176mg/ ml
0.4 / 0.0176 = 22.727 ml (Lucrin needed for a single 0.4mg dose)
50 - 22.727 = 27.273 ml (PBS needed per mouse dose)
For 50 doses:
22.727 x 50 = 1136.35 ml (Lucrin)
27.273 x 50 = 1363.65 ml (PBS)
Warning: Lucrin does not stay in suspension well. When giving IVs draw several doses up into syringe at a time. Don't fill the syringe (take say 3 doses at a time) and don’t leave it in the syringe to settle for a long time or it will clog the needle.