Immunohistochemistry - Thymus architecture MTS-10 & 6C3

  • Cut 4-6um sections on a cryostat (-4oC)
  • Draw around sections with a wax pen
  • Air dry briefly and cover in foil. Place in fridge for at least 30mins
  • Add 30uL MTS-10 and leave for 20mins
  • 2x PBS washes (Place in 1x PBS for 5 minutes each wash)
  • Add 30uL Alexa alpha-rat 568 and leave for 20mins
  • 1x PBS Wash
  • Add 30uL Normal rat serum and leave for 10mins
  • Flick NRS into sink
  • Add 30uL 6C3-FITC and leave for 20mins
  • 1x PBS wash
  • Place a drop of mounting fluid onto a coverslip and place on section slide