Ethidium Bromide/Acridine Orange

 

For discrimination of live from dead cells on the basis of membrane integrity.

•Acridine orange fluoresces GREEN --> LIVE cells.

•Ethidium bromide fluoresces orange (intercalates with DNA) --> DEAD cells.

 

From powder:

 

Working conc for cell viabilities:

Take 1 ml & make up to 100 ml in PBS

 

From solutions: