Embeding of Sensitive Ags

 

MeOH/Tannic acid blocks

Processing tissue for Paraffin embedding

  • Wash tissue in PBS as per usual
  • Trim blocks free of adipose tissue
  • Place blocks in absolute methanol supplemented with 0.5% tannic acid, leave 4˚C O/N
  • 3 X 10 min incubations in absolute methanol
  • 3 X 10 min incubations in absolute ethanol
  • 2 X 30 min in histosol
  • 1 X 60 min in histosol
  • 2 X 2 hr in wax ( place directly into wax chambers )
  • embed