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Concentrated Phosphate Buffered Saline

(pH 7.2 at 1 x)


10 x PBS (Mouse Tonicity)

For 4L:

  • NaCl 348g
  • NaH2PO4.2H2O 25g
  • Na2HPO4 *(anhydrous) 91.0g


* or Na2HPO4.2H2O 114g

or Na2HPO4.12H2O 229.3g


For 20L:

  • NaCl (870g in 5L) x 2
  • NaH2PO4.2H2O 125g in 5L
  • Na2HPO4 * (anhydrous) 454.7g in 5L

Add together to make 20L


* or Na2HPO4.2H2O 570g

or Na2HPO4.12H2O 1146.4g


10 x PBS (Human Tonicity)

For 4L:

  • NaCl 340g
  • NaH2PO4.2H2O 15.6g
  • Na2HPO4* (anhydrous) 42.8g


* or Na2HPO4.2H2O 53.65g

or Na2HPO4.12H2O 107.9g


N.B. Store at room temperature; do not autoclave or refridgerate